E. coli BL21

It is an exemplary host for the expression of recombinant proteins. As an E. coli B strain, it further lacks the major protease, encoded by the lon gene, catalyzing the endoproteolytic cleavage of damaged and recombinant proteins in the cell
大肠杆菌 BL21

订购信息	E. coli BL21: E. coli B F-, ompT, hsdS (rB-, mB-), gal, dcm.
应用	Bacterial strain for the expression of GST fusion protein
基因型	The BL21 strain is derived from E. coli B. The genotype is F-, ompT, hsdS (rB -, mB -), gal, dcm
生长条件	Resuspend lyophilized cultures in 1 ml of LB medium*. Grow overnight at 37°C before plating onto LB agar plates
储存条件	-20°C, Mix equal volumes of stationary phase culture (grown in LB medium) and glycerol. Store at -70°C. Revive frozen glycerol stocks of BL21 by streaking onto LB agar plates
用途	The protease-minus nature of BL21 makes it useful as an expression host. Since BL21 does not transform well, use an alternate strain for cloning and maintenance of the vector

参考编号: 27154201

产品代码: p-03233

SKU： 27154201 分类：细胞培养, 细胞培养试剂耗材, 培养基补料

¥1,194.41

It is an exemplary host for the expression of recombinant proteins. As an E. coli B strain, it further lacks the major protease, encoded by the lon gene, catalyzing the endoproteolytic cleavage of damaged and recombinant proteins in the cell

A protease-deficient E. coli host strain for optimal expression of recombinant proteins

High-level expression of a variety of recombinant proteins

Defective in E. coli strain B, and wild-type restriction systems to permit high efficiency transformation with unmodified plasmid DNA

Verified by RAPD fingerprint analysis using Ready-To-Go RAPD Analysis Kit
Provided in lyophilized form

Well suited for use with the GST Gene Fusion System, E. coli BL21 is an exemplary host for the expression of recombinant proteins (1). As an E. coli B strain, it further lacks the major protease, encoded by the lon gene, catalyzing the endoproteolytic cleavage of damaged and recombinant proteins in the cell (2).