ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins
Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol
Exclusion limit (Mr) (globular proteins)
~3 × 10⁷
Ion Exchanger Type
Weak anion exchanger
Ionic Capacity
0.13-0.18 mmol Cl-/ml medium
Ligand
Diethylaminopropyl
Ligand Coupling Method
Ether linkages and hydrophilic spacer arm
Matrix
cross-linked agarose, 4%, spherical
Particle size, d50V
~90 µm
Pressure/flow characteristics
≥200 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water).
Storage
4 to 30°C, 20% Ethanol
pH ligand fully charged
Below 9
pH stability, CIP
2–14
pH stability, operational
3–13
：500mL
¥10,145.00
17128710
产品详情
参考编号：17128710
产品代码：p-00911
：25mL
ANX Sepharose 4 Fast Flow (High Sub), 25 mL
BioProcess resin
Yes
Chemical stability
Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol
Exclusion limit (Mr) (globular proteins)
~3 × 10⁷
Ion Exchanger Type
Weak anion exchanger
Ionic Capacity
0.13-0.18 mmol Cl-/ml medium
Ligand
Diethylaminopropyl
Ligand Coupling Method
Ether linkages and hydrophilic spacer arm
Matrix
cross-linked agarose, 4%, spherical
Particle size, d50V
~90 µm
Pressure/flow characteristics
≥200 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water).
Storage
4 to 30°C, 20% Ethanol
pH ligand fully charged
Below 9
pH stability, CIP
2–14
pH stability, operational
3–13
：25mL
¥2,881.00
17128760
产品详情
：60L
ANX Sepharose 4 Fast Flow (High Sub), 60 L
BioProcess resin
Yes
Chemical stability
Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol
Exclusion limit (Mr) (globular proteins)
~3 × 10⁷
Ion Exchanger Type
Weak anion exchanger
Ionic Capacity
0.13-0.18 mmol Cl-/ml medium
Ligand
Diethylaminopropyl
Ligand Coupling Method
Ether linkages and hydrophilic spacer arm
Matrix
cross-linked agarose, 4%, spherical
Particle size, d50V
~90 µm
Pressure/flow characteristics
≥200 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water).
Storage
4 to 30°C, 20% Ethanol
pH ligand fully charged
Below 9
pH stability, CIP
2–14
pH stability, operational
3–13
：60L
—
17128705
产品详情
：10L
ANX Sepharose 4 Fast Flow (High Sub), 10 L
BioProcess resin
Yes
Certificate of Analysis
Yes
Chemical stability
Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol
Exclusion limit (Mr) (globular proteins)
~3 × 10⁷
Ion Exchanger Type
Weak anion exchanger
Ionic Capacity
0.13-0.18 mmol Cl-/ml medium
Ligand
Diethylaminopropyl
Ligand Coupling Method
Ether linkages and hydrophilic spacer arm
Matrix
cross-linked agarose, 4%, spherical
Particle size, d50V
~90 µm
Pressure/flow characteristics
≥200 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water).
Storage
4 to 30°C, 20% Ethanol
pH ligand fully charged
Below 9
pH stability, CIP
2–14
pH stability, operational
3–13
：10L
—
17128704
产品详情
：5L
ANX Sepharose 4 Fast Flow (High Sub), 5 L
BioProcess resin
Yes
Certificate of Analysis
Yes
Chemical stability
Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol
Exclusion limit (Mr) (globular proteins)
~3 × 10⁷
Ion Exchanger Type
Weak anion exchanger
Ionic Capacity
0.13-0.18 mmol Cl-/ml medium
Ligand
Diethylaminopropyl
Ligand Coupling Method
Ether linkages and hydrophilic spacer arm
Matrix
cross-linked agarose, 4%, spherical
Particle size, d50V
~90 µm
Pressure/flow characteristics
≥200 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20°C using buffers with the same viscosity as water).
Storage
4 to 30°C, 20% Ethanol
pH ligand fully charged
Below 9
pH stability, CIP
2–14
pH stability, operational
3–13
：5L
—
产品详情
ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins
Weak anion exchanger with different selectivity compared with traditional ion exchangers.
Applicable for separation of high molecular weight proteins.
Developed in co-operation with leading large-scale pharmaceutical manufacturers.
High chemical stability allows for well proven CIP and sanitization protocols.
The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
ANX Sepharose Fast Flow (high sub) is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger resin with tertiary amine groups attached to the base matrix Sepharose 4 Fast Flow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose 4 Fast Flow, is a highly cross-linked, 4% agarose derivative with high chemical and physical stability and broad separation range. ANX Sepharose 4 Fast Flow (high sub) has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols.
As member of the BioProcess resin range, ANX Sepharose 4 Fast Flow (high sub) meets industrial demands with security of supply and comprehensive technical and regulatory support.
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APPLICATION NOTE
EN
1.Cleaning procedures for ÄKTAprocess system external surfaces
