GST 96-Well Detection Module
Product has been discontinued and is not replaced.
| Application | Used for detection of GST fusion proteins |
| Storage conditions | -15 – -30°C |
| Kit contents | Five 96-well strip plates (12 strips of 8 wells each). Each well is coated with Anti- GST Antibody, blocked, and dried. Each GST 96-Well Detection Plate is stored in a resealable foil pouch. HRP/Anti-GST Conjugate: Horseradish peroxidase conjugated to goat polyclonal anti-GST IgG. Sufficient HRP/Anti-GST Conjugate for twenty 96-well GST detection assays is provided. rGST Protein: 20 μl of a 5 mg/ml solution of Glutathione Sepharose 4B affinity purified recombinant glutathione S-transferase test protein |
询价
产品详情
- Enables sensitive detection of GST fusion proteins
- The wells of each plate are coated with purified Anti-GST Antibody to capture GST fusion proteins and are preblocked to provide a low background
- Contains five microplates, horseradish peroxidase (HRP), conjugated Anti-GST Antibody, and recombinant GST protein.
GST 96-Well Detection Module permits rapid, sensitive determination of glutathione S-transferase (GST) fusion proteins in a variety of samples. Clarified lysates or intermediate purification fractions can be applied directly into the wells of GST 96-Well Detection Plates. GST fusion proteins are captured by specific binding to Anti-GST Antibody that is immobilized on the walls of each well. Captured GST fusion proteins are detected with HRP/ Anti-GST Conjugate provided in the module. Standard curves for quantification of fusion proteins can be constructed using recombinant GST, which is included as a control. The GST 96-Well Detection Module can also be used with antibody specific for a GST fusion partner to screen as many as 96 samples per plate to identify clones expressing the desired GST fusion protein.